On targeting Morpholinos for splicing:
- Splicing outcomes: targeting for exon skipping or intron inclusion
- Assessing activity of a splice-modifying Morpholino
- Which splice junction to target, predicting cryptic splice sites, and doing the two-non-overlapping oligo specificity experiment
- Example of splice enhancer targeting
- Formatting sequence information for emails (splice modifying oligo designs)
- Targeting internal exon caused intron inclusion: example of a less-common outcome
- An i1e2 Morpholino triggering intron 1 retention: unusual splice outcome
- Morpholino-based skipping of an exon caused inclusion of another exon, following that transcipt's pattern of normal alternative splicing
On targeting noncoding RNAs:
On specificity controls:
- Guidelines for morpholino use in zebrafish
- BLAST homology: screening predicted specificity
- On my (low) opinion of five mispair specificity controls
- Confirming specificity
- BLAST homology and specificity controls
- miRNA targeting and second oligo specificity controls
On negative controls:
Bearing on p53 knockdowns in zebrafish:
On duration of Morpholino effect:
- Change in Morpholino activity over time
- Timing of protein measurement in a translation-blocking experiment
- Morpholino duration of effect
On handling Morpholinos and keeping them in solution:
On measuring Morpholino concentration:
On mutants and morphants:
- Genetic compensation induced by deleterious mutations but not gene knockdowns
- Fish mutant, where is thy phenotype?
- Off-target Morpholino interaction reported based on mutant and 6ng dose
On getting Morpholino into cultured cells:
On unusual routes of administration of Morpholinos:
- Oocyte microinjection in situ (OMIS): Morpholinos in zebrafish oocytes in the ovary
- Knockdowns in mosquito guts
- Intranasal administration in mice of peptide-conjugated Morpholinos
- Intestinal delivery to zebrafish from fish water
- Bathing zebrafish embryos with Vivo-Morpholinos
- Abdominal injection of Vivo-Morpholino in fish for oocyte knockdown
- Combining Vivo-Morpholinos & Endo-Porter in a hard-to-transfect cell line
- Cardiac Ventricular Injection in Zebrafish Larva
On Morpholino-based therapeutics:
On Morpholinos as probes:
On purifying and analyzing Morpholinos by HPLC and detecting Morpholinos in biological samples:
Books focused mostly or entirely on Morpholino oligos:
Link to the whole blog: