Citations of papers with Morpholinos blocking mRNA localization elements are here.
Morpholino oligos have been shown effective for blocking localization elements on mRNAs, inhibiting binding of the RNA recognition proteins needed for directed movement of the mRNAs.
References
Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S. mRNA location and translation rate determine protein targeting to dual destinations. Mol Cell. 2023 Jul 20:S1097-2765(23)00514-2. doi: 10.1016/j.molcel.2023.06.036. Online ahead of print. cell culture: MDA-MB-231 Moriarty RA, Mili S, Stroka KM. RNA Localization in Confined Cells Depends on Cellular Mechanical Activity and Contributes to Confined Migration. iScience. 2022;[Epub ahead of print] doi:10.2139/ssrn.3878411 Chrisafis G, Wang T, Moissoglu K, Gasparski AN, Ng Y, Weigert R, Lockett SJ, Mili S. Collective cancer cell invasion requires RNA accumulation at the invasive front. Proc Natl Acad Sci U S A. 2020;[Epub ahead of print] doi:10.1073/pnas.2010872117 Moissoglu K, Stueland M, Gasparski AN, Wang T, Jenkins LM, Hastings ML, Mili S. RNA localization and co-translational interactions control RAB13 GTPase function and cell migration. EMBO J. 2020 Sep 18:e2020104958. doi: 10.15252/embj.2020104958. Online ahead of print Gilligan PC, Kumari P, Lim S, Cheong A, Chang A, Sampath K. Conservation defines functional motifs in the squint/nodal-related 1 RNA dorsal localization element. Nucleic Acids Res. 2011 Apr 1;39(8):3340-9. Epub 2010 Dec 10. Rabinowitz JS, Lambert JD. Spiralian quartet developmental potential is regulated by specific localization elements that mediate asymmetric RNA segregation. Development. 2010 Nov 1. [Epub ahead of print] Koebernick K, Loeber J, Arthur PK, Tarbashevich K, Pieler T. Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma. Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16148-53. Epub 2010 Aug 30 Arthur PK, Claussen M, Koch S, Tarbashevich K, Jahn O, Pieler T. Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis. J Biol Chem. 2009 Jul 24;284(30):19982-92. Epub 2009 May 20.
Morpholino oligos have been shown effective for blocking localization elements on mRNAs, inhibiting binding of the RNA recognition proteins needed for directed movement of the mRNAs.
References
Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S. mRNA location and translation rate determine protein targeting to dual destinations. Mol Cell. 2023 Jul 20:S1097-2765(23)00514-2. doi: 10.1016/j.molcel.2023.06.036. Online ahead of print. cell culture: MDA-MB-231 Moriarty RA, Mili S, Stroka KM. RNA Localization in Confined Cells Depends on Cellular Mechanical Activity and Contributes to Confined Migration. iScience. 2022;[Epub ahead of print] doi:10.2139/ssrn.3878411 Chrisafis G, Wang T, Moissoglu K, Gasparski AN, Ng Y, Weigert R, Lockett SJ, Mili S. Collective cancer cell invasion requires RNA accumulation at the invasive front. Proc Natl Acad Sci U S A. 2020;[Epub ahead of print] doi:10.1073/pnas.2010872117 Moissoglu K, Stueland M, Gasparski AN, Wang T, Jenkins LM, Hastings ML, Mili S. RNA localization and co-translational interactions control RAB13 GTPase function and cell migration. EMBO J. 2020 Sep 18:e2020104958. doi: 10.15252/embj.2020104958. Online ahead of print Gilligan PC, Kumari P, Lim S, Cheong A, Chang A, Sampath K. Conservation defines functional motifs in the squint/nodal-related 1 RNA dorsal localization element. Nucleic Acids Res. 2011 Apr 1;39(8):3340-9. Epub 2010 Dec 10. Rabinowitz JS, Lambert JD. Spiralian quartet developmental potential is regulated by specific localization elements that mediate asymmetric RNA segregation. Development. 2010 Nov 1. [Epub ahead of print] Koebernick K, Loeber J, Arthur PK, Tarbashevich K, Pieler T. Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma. Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16148-53. Epub 2010 Aug 30 Arthur PK, Claussen M, Koch S, Tarbashevich K, Jahn O, Pieler T. Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis. J Biol Chem. 2009 Jul 24;284(30):19982-92. Epub 2009 May 20.