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Delivery of Morpholinos by irradiation

Patent:
Morpholino oligonucleotides useful in cancer treatment
https://patents.google.com/patent/US11679121B2/en
Includes delivery of Morpholinos by irradiation

Thursday, July 13, 2023 - 15:33
(Methods Cell Biol):Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish

Cardenas-Rodriguez M, Drummond IA. The challenge of dissecting gene function in model organisms: Tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish. Methods Cell Biol. 2023;176:1-25. doi: 10.1016/bs.mcb.2022.12.019. Epub 2023 Jan 27.
https://www.sciencedirect.com/science/article/abs/pii/S0091679X22002072

Thursday, May 11, 2023 - 15:29
I have an article in Pharma's Almanac - Morpholino stories

Morpholino article in Pharma's Almanac

Friday, May 5, 2023 - 15:34
(Review) Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy

Recent Trends in Antisense Therapies for Duchenne Muscular Dystrophy.
Wilton-Clark H, Yokota T. Pharmaceutics. 2023; 15(3):778. https://doi.org/10.3390/pharmaceutics15030778
https://www.mdpi.com/1999-4923/15/3/778

Thursday, March 30, 2023 - 14:23
Morpholinos shorter than 25 bases

A Morpholino user, accustomed to using 25-base Morpholinos in Xenopus laevis, was concerned about a design she received for a 20-base Morpholino. She wrote to ask if we had designed many short oligos and if they worked well. Here is my response.

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Wednesday, February 15, 2023 - 11:13
Molecular dynamics of aqueous Morpholino oligos

Maksudov F, Kliuchnikov E, Pierson D, Ujwal ML, Marx KA, Chanda A, Barsegov V. Therapeutic Phosphorodiamidate Morpholino Oligonucleotides, an RNA mimic: Physical Properties, Solution Structures, and Folding Thermodynamics. Mol Ther Nucleic Acids. 2023;[Epub] doi:10.1016/j.omtn.2023.02.007

https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S21...(23)00027-6

Wednesday, February 15, 2023 - 10:15
Discussion of RNA change on adding translation blocking Morpholino

From: ****
Sent: Wednesday, January 18, 2023 10:17 AM
To: Jon Moulton
Subject: Translation-blocking Vivo-MO reduces gene expression on the level of mRNA?

Hello Jon,

Wednesday, January 18, 2023 - 12:35
History: exon skipping

Lim, K.R.Q., Yokota, T. (2018). Invention and Early History of Exon Skipping and Splice Modulation. In: Yokota, T., Maruyama, R. (eds) Exon Skipping and Inclusion Therapies. Methods in Molecular Biology, vol 1828. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8651-4_1
https://link.springer.com/protocol/10.1007/978-1-4939-8651-4_1

Tuesday, January 17, 2023 - 11:03
Master’s thesis based on microarray analysis of WT, Morphant and rescue fish

Master’s thesis based on microarray analysis of WT, Morphant and rescue fish
Tuttle, Matthew Alan (2017)
In silico analysis of zebrafish leptin-a knockdown gene expression data reveals enrichment for metabolic and developmental pathways including morpholino artifacts
https://etd.ohiolink.edu/apexprod/rws_olink/r/1501/10?clear=10&p10_acces...

Monday, January 16, 2023 - 11:32
Vivo-Morpholino uptake by zebrafish larvae at 4dpf

Liang H, Li Y, Li M, Zhou W, Chen J, Zhang Z, Yang Y, Ran C, Zhou Z. The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection. Front Immunol. 2022 Nov 3;13:1031962. doi: 10.3389/fimmu.2022.1031962. eCollection 2022.
https://www.frontiersin.org/articles/10.3389/fimmu.2022.1031962/full

Monday, November 21, 2022 - 09:48

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