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|Mutant-morphant mismatch but no genetic compensation||
Zebrafish Chromosome 14 Gene Differential Expression in the fmr1 h u2787 Model of Fragile X Syndrome.
|Friday, June 18, 2021 - 08:14|
|Morphant vs mutant: "...this knockout line is deemed unsuitable for our study."||
Here’s a nice description of why, in this study, morphants are preferable to mutants.
|Friday, June 11, 2021 - 11:53|
|Review of neurodegenerative disease and splice modulation||
Neurodegenerative diseases: a hotbed for splicing defects and the potential therapies.
|Monday, May 24, 2021 - 13:32|
|Therapeutic Modulation of RNA Splicing in Malignant and Non-Malignant Disease (Review)||
Therapeutic Modulation of RNA Splicing in Malignant and Non-Malignant Disease.
|Monday, May 17, 2021 - 11:33|
|SARS-CoV-2 RNA targets for steric blocking||
Potantial targets for steric blocking on the SARS-CoV-2 genome and transcripts.
The SARS-CoV-2 RNA interactome.
|Monday, May 17, 2021 - 10:23|
|Positive control, human and mouse splice modification||
Flynn LL, Mitrpant C, Adams A, Pitout IL, Stirnweiss A, Fletcher S, Wilton SD. Targeted SMN Exon Skipping: A Useful Control to Assess In Vitro and In Vivo Splice-Switching Studies. Biomedicines. 2021;9(5):552. doi:10.3390/biomedicines9050552
|Friday, May 14, 2021 - 11:52|
|MO knockdown of ectopic GFP in axlotl||
There are few papers reporting using a Morpholino to directly target GFP in vivo; here is one example.
|Friday, April 23, 2021 - 11:21|
|Oligo types, therapeutic Morpholinos, upregulation with Morpholinos||
Here are some types of oligos sorted by their mechanisms.
RNase-independent (steric blocking oligos)
About the Morpholino drugs
|Thursday, April 15, 2021 - 09:24|
|Review of oligo delivery for therapeutics||
Hammond SH et al. Delivery of oligonucleotide-based therapeutics: challenges and opportunities. Embo Mol Med. 2021:e13243. doi: 10.15252/emmm.202013243
|Wednesday, April 7, 2021 - 09:48|
|Review of mutant-morphant discrepency & genetic compensation||
Salanga CM, Salanga MC. Genotype to Phenotype: CRISPR Gene Editing Reveals Genetic Compensation as a Mechanism for Phenotypic Disjunction of Morphants and Mutants. Int J Mol Sci. 2021;22(7),3472. doi:10.3390/ijms22073472
|Monday, March 29, 2021 - 13:39|