Download Protocols, Publications and Useful Documents

Table of Contents

Links to Resources

Protocols and Product Information

• Designing and Ordering Morpholinos & Vivo-Morpholinos


• Using Morpholinos & Vivo-Morpholinos


• Endo-Porter protocol


• Scrape delivery protocol


• MSDS files


• Determining the concentration of a Morpholino stock solution


• Using a NanoDrop spectrometer to determine the concentration of a Morpholino stock solution Note: when testing drop-based spectrometers quantifying Morpholino solutions, we have had difficulties with reproducibility and when testing drop-based spectrometers alongside spectrometers using 1 cm quartz cells, the data did not agree. We recommend using 1 cm quartz cells for quantifying Morpholinos.


• Assaying efficacy of delivery into cells by fluorescence


• Generating a sulfhydryl from a Morpholino bearing a disulfide modification


• Amaxa application note describing nucleofection of Morpholinos into cultured cells


• Excitation and emission profiles for our fluorescent modifications: carboxy-fluorescein (green) and lissamine (red)


• Excitation and emission profiles for our fluorescent modification: Gene Tools Blue (blue)


• Notes on storage and lyophilization of Morpholinos

Critical Papers and Tutorials

• A brief introduction to Morpholino antisense


• Summerton's paper Custom Cocktail for Curing any Cancer: A Strategy for Destroying any Cancer without Harming the Patient


• Moulton's paper Guide for Morpholino Users: Toward Therapeutics


• Jim Summerton's 2005 paper discussing Morpholinos for pharmaceutical research in Innovations in Pharmaceutical Technology


• Kloosterman, Lagendijk, Ketting, Moulton, Plasterk 2007 paper demonstrating blocking miRNA maturation with Morpholinos

Splice modification resources


• Discussion on the advantages of using Morpholinos for altering pre-mRNA splicing


• Morcos 2007 paper describing splice site targeting


• Draper, Morcos, Kimmel 2001 paper first showing successful splice-blocking in zebrafish


• Madsen, Morcos, Mendelsohn, Gitlin. Menkes disease model; splice modification with a Morpholino walking experiment demonstrating correlation of activity and target position.

Key Publications

• Summerton J (2007). Morpholino, siRNA, and S-DNA Compared: Impact of Structure and Mechanism of Action on Off-Target Effects and Sequence Specificity. Med Chem. 7(7):651-660.


• Summerton JE. (2005) Endo-Porter: A Novel Reagent for Safe, Effective Delivery of Substances into Cells. Ann N Y Acad Sci.;1058:62-75


• Summerton (1999). Morpholino Antisense Oligomers: The case for an RNase H-independent structural type. Biochimica et Biophysica Acta 1489, 141.


• Summerton & Weller (1997). Morpholino antisense oligomers: design, preparation and properties. Antisense Nuc. Acid Drug Dev.7 , 187.


• Summerton, Stein, Huang, Matthews, Weller, Partridge (1997). Morpholino and phosphorothioate antisense oligomers compared in cell-free and in-cell systems. Antisense Nuc. Acid Drug Dev.7 , 63.


• Partridge, Vincent, Matthews, Puma, Stein, Summerton (1996). A simple method for delivering Morpholino antisense oligos into the cytoplasm of cells. Antisense Nuc. Acid Drug Dev. 6, 169.

Ordering Morpholinos

• Fax order form

Shipping documents

• Document describing Morpholino oligos for customs


• Chinese language document describing Morpholino oligos for Chinese customs

Presentations

• Jim Summerton's Powerpoint presentation on the development of Morpholino delivery techniques at OSU Biochem/Biophysics, November 2012.


• This 2023 interview with Jon Moulton on Pharma's Almanac discusses using Morpholinos to alter splicing.


• Jon Moulton's Powerpoint presentation for his lecture to a non-technical audience in Philomath, Oregon in 2008 at the Marys Peak Natural Resources Interpretive Center


• Jon Moulton's Powerpoint presentation for Western China 2005


• 2005 Poster showing research applications of Morpholino oligos: targets, organisms, delivery strategies

Selected posts from Jon's Blog

• This link to selected posts from Jon's blog shows a partial table of contents and provides quick access to commonly used discussions and explanations.

Audio Notes (mp3 format)

• A set of mp3 files describing techniques involving Morpholino oligos can be downloaded from another page (click this link).

Pharma's Almanac article

• Morpholino article in Pharma's Almanac

Materials for Special Delivery experiments

The Special Delivery system was a step in the development of Morpholino delivery systems for cell culture. Morpholinos are now typically delivered to cultured cells by the use of Endo-Porter to deliver unmodified Morpholinos, by electroporation, or by the use of Vivo-Morpholinos in cultures. We are retaining this section on our website to provide historical context for published accounts of work using the Special Delivery system. We no longer manufacture or sell Special Delivery DNA-Morpholino heteroduplexes or EPEI delivery reagent.

• Protocol for making a Special Delivery oligo.


• Special Delivery protocol for delivering Morpholino-DNA heteroduplexes into cell cultures with EPEI.


• Paper describing Special Delivery: method for Morpholino delivery into cultured cells, including selection of DNA oligos, hybridization of DNA with Morpholinos and delivery of Morpholino-DNA heteroduplexes with ethoxylated polyethylenimine (EPEI)

---

Copies of publications from Antisense and Nucleic Acid Drug Development are provided with permission from the publisher:

---

An appropriate viewer is required to open Portable Document Format (PDF) files. Some suggested viewers (all available free-of-charge) for various platforms are:

Linux/UNIX: Evince
MacOS X: Apple Preview
MacOS, pre-X: Adobe Reader
Windows: Adobe Reader