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Jon's Blog
| Post date | ||
|---|---|---|
| J David Lambert's notes on oligo handling, autoclaving |
From the paper: |
Friday, July 8, 2016 - 09:13 |
| Morpholino special issue: Journal of Drug DDD |
There's a Morpholino special issue of the Austin Journal of Drug Discovery, Development and Delivery. |
Thursday, July 7, 2016 - 07:29 |
| Link to an antimicrobial antisense review (Sully & Geller) |
Antisense antimicrobial therapeutics. Sully EK, Geller BL. Curr Opin Microbiol. 2016 Jun 29;33:47-55. doi: 10.1016/j.mib.2016.05.017. [Epub ahead of print] Review. http://www.sciencedirect.com/science/article/pii/S1369527416300807 (open-access) |
Wednesday, July 6, 2016 - 14:19 |
| Tm of a Morpholino - Effective? Specific? |
A Morpholino user asked me about his oligo design, which I had marked with a Tm of 82.5°C. He wondered about efficacy and specificity of such an oligo. Here's my response. |
Tuesday, June 14, 2016 - 10:58 |
| Review: Splice-switching antisense oligonucleotides as therapeutic drugs |
Here's a review I'd like to keep track of. Splice-switching antisense oligonucleotides as therapeutic drugs. Havens MA, Hastings ML. Nucleic Acids Res. 2016 Jun 10. pii: gkw533. [Epub ahead of print] http://nar.oxfordjournals.org/content/early/2016/06/10/nar.gkw533.long |
Monday, June 13, 2016 - 14:20 |
| Timing of protein measurement in a translation-blocking experiment |
This was written to a researcher in response to his question about timing of assays in a translation-blocking experiment. ----------------------------------------------- |
Friday, May 20, 2016 - 12:56 |
| Immunology citations 2014 to early 2016 |
I'm headed for the American Association for Immunologists conference (AAI 2016). I assembled a citation list of many of the immunology papers published over the last couple of years. Here it is. Some Morpholino Citations for Immunology (AAI 2016) Furukawa R, Tamaki K, Kaneko H. Two macrophage migration inhibitory factors regulate starfish larval immune cell chemotaxis. Immunol Cell Biol. 2016 Apr;94(4):315-21. doi: 10.1038/icb.2016.6. Epub 2016 Feb 2. Patiria pectinifera (sea star) |
Thursday, May 12, 2016 - 15:19 |
| Guide for Morpholino Users: Toward Therapeutics |
My open-access review paper was just published: |
Wednesday, May 4, 2016 - 08:55 |
| Brief discussion of Morpholino efficacy, specificity & controls from review in J. Cardiovasc. Dev. Dis. |
Review Daniel R. Brown, Leigh Ann Samsa, Li Qian, Jiandong Liu. http://www.mdpi.com/2308-3425/3/2/13/htm Keyword search "Morpholino" or go to section "3.1.2. Morpholinos" for a brief discussion of Morpholino efficacy, specificity and controls. |
Monday, April 11, 2016 - 11:45 |
| GFP targeted directly with a Morpholino - example |
Makino S, Mishima Y, Inoue K, Inada T. Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast. J Biol Chem. 2015 Mar 27;290(13):8331-47. doi: 10.1074/jbc.M114.615088. Epub 2015 Feb 5. "To address this question, we injected GFP reporter mRNA into zebrafish embryos and then blocked its translation using a morpholino oligomer (ATG MO) that specifically masks the start codon of the GFP ORF." |
Friday, April 1, 2016 - 14:11 |
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