Depletion of Foxk transcription factors causes genome-wide transcriptional misregulation and developmental arrest in zebrafish embryos

no abstract available Figure 1. Morpholino (MO) knockdown of foxk1/foxk2a/foxk2b and transcriptional profiling of triple foxkmorphants: A) Upper panels:Zebrafish larvae (72 hpf) injected with morpholinos targeting single foxk transcripts. Lowerpanels: triple (foxk1/foxk2a/foxk2b) MO and rescue with full length (+ hfoxk1 RNA) or mutated (+ hfoxk1-mut) humanfoxk1 transcripts, at 24hpf. Lower right panel: Hierarchical clustering of embryo numbers corresponding to: triple MO,triple MO with full length foxk1 transcript, triple MO with mutated foxk1 transcript, and wild type, divided into phenotypeseverity groups (weak/wt: no notable developmental delay; mild: minor developmental delay, trunk curvature,pigmentation issues; strong: severe developmental delay, nervous system defects, shortened body axes). B) Similaritydistance matrix of RNA-seq data (counts) corresponding to wild type (wt) and triple morpholino (MO) conditions. C) MAplot displaying upregulated and downregulated genes (red dots) caused by triple Foxk MO. D) Expression profiles (TPM)of previously described Foxk targets (fbxo32, ulk1b) in wt and triple MO conditions. E) Gene ontology analysis of themost significantly (n=500) upregulated and downregulated genes in the triple MO condition.