This is an older review (2008), but has a nice discussion of Morpholinos and controls. Photo-Morpholino and Vivo-Morpholino use in zebrafish are not addressed. The newer strategy of using Morpholinos in CRISPR mutants for specificity control is more recent than this paper, as is the understanding of gene compensation in mutants versus relatively uncompensated phenotypes from knockdowns triggered by Morpholino microinjections.
Skromne I, Prince VE.
Current perspectives in zebrafish reverse genetics: moving forward.
Dev Dyn. 2008 Apr;237(4):861-82. doi: 10.1002/dvdy.21484. Review.
Add new comment