Citation:
Methods Mol Biol. 2018;1742:205-211. doi: 10.1007/978-1-4939-7665-2_18
Abstract:
In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
Epub:
Not Epub
Link to Publication:
https://link.springer.com/protocol/10.1007%2F978-1-4939-7665-2_18
Organism or Cell Type:
zebrafish
Delivery Method:
microinjection