Citation:
J Cell Sci. 2017;[Epub ahead of print] doi:10.1242/jcs.206565
Abstract:
Multiciliated cells (MCCs) drive fluid flow in diverse tubular organs and are essential for the development and homeostasis of the vertebrate central nervous system, airway, and reproductive tracts. These cells are characterized by dozens or hundreds of motile cilia that beat in a coordinated and polarized manner. In recent years, genomic studies have not only elucidated the transcriptional hierarchy for MCC specification, but also identified myriad new proteins that govern MCC ciliogenesis, cilia beating, or cilia polarization. Interestingly, this burst of genomic data has also highlighted the obvious importance of proteins with no obvious role in cilia. Understanding the function of proteins with little prior history of study presents a special challenge, especially when faced with large numbers of such proteins. Here, we define the subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology. Functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis.
Epub:
Yes
Link to Publication:
http://jcs.biologists.org/content/early/2017/11/24/jcs.206565
Organism or Cell Type:
Xenopus
Delivery Method:
microinjection