Citation:
Methods Mol Biol. 2017;1648:39-52. doi: 10.1007/978-1-4939-7204-3_4
Abstract:
Regulation of alternative splicing can be harnessed by antisense-based compounds to control gene expression. Antisense-mediated splicing interference has become a valuable molecular tool to modulate endogenous alternative splicing patterns, to correct cryptic or aberrant splicing, to reduce gene expression by triggering nonsense-mediated mRNA decay, and to activate intronic polyadenylation, both in vitro and in vivo. Here, we describe methods to induce and analyze the modulation of RNA processing, using modified splice-switching antisense oligonucleotides, such as phosphorodiamidate morpholino (PMO).
Epub:
Not Epub
Link to Publication:
https://link.springer.com/protocol/10.1007%2F978-1-4939-7204-3_4
Organism or Cell Type:
cell culture