Citation:
Methods Mol Biol. 2017;1565:241-250. doi: 10.1007/978-1-4939-6817-6_20
Abstract:
In vivo delivery of morpholino antisense oligonucleotides (MAO) directly into the uterine lumen of a peri-implantation period pregnant sheep is an effective technique for evaluation of gene products for conceptus development. The highly phagocytic conceptus is undergoing rapid morphological change, thereby the available MAO are readily consumed and delivered to developing cells. Here, we describe the method for preparation and surgical delivery of MAO-Endo-Porter complex to developing ovine conceptus on day 8 postmating. Also outlined are methods for posttreatment sample recovery on day 16 postmating.
Epub:
Not Epub
Link to Publication:
http://www.springer.com/us/book/9781493968152
Organism or Cell Type:
Ovis aries (sheep)
Delivery Method:
Endo-Porter