You are here

In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina

Authors: 
Karunakaran DK, Kanadia R
Citation: 
Methods Mol Biol. 2017;1565:215-227. doi: 10.1007/978-1-4939-6817-6_18
Abstract: 
Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.
Epub: 
Not Epub
Organism or Cell Type: 
mice
Delivery Method: 
electroporation