Citation:
Dev Biol. 2015 Apr 1;400(1):72-81. doi: 10.1016/j.ydbio.2015.01.016. Epub 2015 Jan 31
Abstract:
Precise regulation of Notch signaling is essential for normal vertebrate development. Mind bomb (Mib) is a ubiquitin ligase that is required for activation of Notch by Notch׳s ligand, Delta. Sorting Nexin 5 (SNX5) co-localizes with Mib and Delta complexes and has been shown to directly bind to Mib. We show that microRNA-216a (miR-216a) is expressed in the retina during early development and regulates snx5 to precisely regulate Notch signaling. miR-216a and snx5 have complementary expression patterns. Knocking down miR-216a and/or overexpression of snx5 resulted in increased Notch activation. Conversely, knocking down snx5 and/or miR-216a overexpression caused a decrease in Notch activation. We propose a model in which SNX5, precisely controlled by miR-216a, is a vital partner of Mib in promoting endocytosis of Delta and subsequent activation of Notch signaling.
Epub:
Not Epub
Link to Publication:
http://www.sciencedirect.com/science/article/pii/S0012160615000366
Organism or Cell Type:
zebrafish
Delivery Method:
microinjection