Citation:
Nature. 2016;[Epub ahead of print] doi:10.1038/nature16974
Abstract:
Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed1, 2, 3. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals4, 5. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.
Epub:
Yes
Link to Publication:
http://www.nature.com/nature/journal/vaop/ncurrent/full/nature16974.html
Organism or Cell Type:
cell culture: A1 myoblasts, axolotl
Delivery Method:
electroporation, injection then electroporation