Citation:
Dev Dyn. 2013 Sep;242(9):1101-9. doi: 10.1002/dvdy.23990. Epub 2013 Jul 26
Abstract:
BACKGROUND: PPM1G is a nuclear localized serine/threonine phosphatase implicated to be a regulator of chromatin remodeling, mRNA splicing, and DNA damage. However, its in vivo function is unknown.
RESULTS: Here we show that ppm1g expression is highly enriched in the central nervous system during mouse and zebrafish development. ppm1g(-/-) mice were embryonic lethal with incomplete penetrance after E12.5. Rostral defects, including neural tube and craniofacial defects were observed in ppm1g(-/-) embryos associated with increased cell death in the neural epithelium. In zebrafish, loss of ppm1g also led to neural defects with aberrant neural marker gene expression. Primary fibroblasts from ppm1g(-/-) embryos failed to grow without immortalization while immortalized ppm1g(-/-) fibroblasts had increased cell death upon oxidative and genotoxic stress when compared to wild type fibroblasts.
CONCLUSIONS: Our in vivo and in vitro studies revealed a critical role for PPM1G in normal development and cell survival.
Epub:
Not Epub
Link to Publication:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230483/
Organism or Cell Type:
zebrafish
Delivery Method:
microinjection