Citation:
Nat Commun. 2014 Mar 24;5:3516. doi: 10.1038/ncomms4516
Abstract:
The Eph/ephrin signalling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decrease in ephrinB2 protein levels is observed on the loss of flotillin-1 scaffold protein, a newly identified ephrinB2-binding partner. This dramatic decline in ephrinB2 protein levels on the absence of flotillin-1 expression is specific, and is partly the result of an increased susceptibility to cleavage by the metalloprotease ADAM10. These findings indicate that flotillin-1 regulates ephrinB2 protein levels through ADAM10, and is required for appropriate neural tube morphogenesis in the Xenopus embryo.
Epub:
Not Epub
Link to Publication:
http://www.nature.com/ncomms/2014/140324/ncomms4516/full/ncomms4516.html
Organism or Cell Type:
Xenopus laevis
Delivery Method:
microinjection