Citation:
Nucl Acids Res. 2012;[Epub ahead of print] doi:10.1093/nar/gks840
Abstract:
Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
Organism or Cell Type:
zebrafish
Delivery Method:
Microinjection