Citation:
Mol Biol Cell. 2013 Sep 25. [Epub ahead of print]
Abstract:
Smyd1b is a member of the Smyd family, which is specifically expressed in skeletal and cardiac muscles. Smyd1b plays a key role in thick filament assembly during myofibrillogenesis in skeletal muscles of zebrafish embryos. To better characterize Smyd1b function and its mechanism of action in myofibrillogenesis, we have analyzed the effects of smyd1b knockdown on myofibrillogenesis in skeletal and cardiac muscles of zebrafish embryos. The results showed that knockdown of smyd1b caused a significant disruption of myofibril organization in both skeletal and cardiac muscles of zebrafish embryos. Microarray and quantitative RT-PCR analyses showed that knockdown of smyd1b upregulated heat shock protein 90 (hsp90) and unc45b gene expression. Biochemical analysis revealed that Smyd1b could be coimmunoprecipitated with Hsp90α1 and Unc45b, two myosin chaperones expressed in muscle cells. Consistent with its potential function in myosin folding and assembly, knockdown of smyd1b significantly reduced myosin protein accumulation without affecting their mRNA expression. This likely resulted from increased myosin degradation involving unc45b overexpression. Together, these data support the idea that Smyd1b may work together with myosin chaperones to control myosin folding, degradation and assembly into sarcomeres during myofibrillogenesis.
Epub:
Yes
Organism or Cell Type:
zebrafish
Delivery Method:
Microinjection