Shen H-w, Scofield MD, Boger H, Hensley M, Kalivas PW. Synaptic Glutamate Spillover Due to Impaired Glutamate Uptake Mediates Heroin Relapse. J Neurosci. 2014;34(16):5649-57 doi:10.1523/JNEUROSCI.4564-13.2014 http://www.jneurosci.org/content/34/16/5649.abstract Vivo-Morpholinos targeting the glial excitatory amino acid transporter 2 (GLT-1) were administered to adult male Sprague Dawley rats by infusion through microdialysis guide cannulas (26 ga) implanted bilaterally in their nucleus accumbens (position details in paper). Vivo-Morpholino stocks (0.5 mM) were diluted 50-fold in PBS. 1 ul was infused at 0.5 ul/min though 33ga microinjectors extending 2mm below the cannula, with the injectors left in place for 60 seconds after infusion was completed. Injections were done once per day for three days and GLT-1 assessed from tissue taken seven days later. Their protocol was shown to reduce GLT-1 expression by 56% (+/- 10%) in the nucleus accumbens seven days after the last infusion.