Using morpholinos for gene knockdown in Giardia intestinalis

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either GFP or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter of morpholino efficacy for targets lacking specific antibodies.