Citation:
Nippon Kagakkai Koen Yokoshu 2003; 83(2):1126 (Japanese)
Abstract:
Triplex DNA is usually formed through the sequence-specific interaction of a single-stranded homopurine or homopyrmidine triplex-forming oligonucleotide (TFO) with the major groove of homopurine-homopyrmidine stretch in duplex DNA. Pyrmidine motif triplex involving homopyrmidine TFO is stable only at acidic pH. However, pyrmidine motif triplex needs to be stabilized at neutral pH for its application to artificial control of gene expression. This subject will present that morpholino modification of TFO stabilized the pyrmidine motif triplex at neutral pH, and the stabilization mechanism was examined by thermodynamic and kinetic analyses.