This paper presents an example of a Morpholino off-target effect shown by testing the Morpholino in a CRISPR mutants.
Jiang Z, Carlantoni C, Allanki S, Ebersberger I, Stainier DYR. Tek/Tie2 is not required for cardiovascular development in zebrafish. Development. 2020 Sep 14:dev.193029. doi: 10.1242/dev.193029. Online ahead of print.
https://dev.biologists.org/content/early/2020/09/12/dev.193029
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