Citation:
Cells Dev. 2025 Jan 2:203993. doi: 10.1016/j.cdev.2024.203993. Epub ahead of print. PMID: 39755275
Abstract:
The temporal control of mitotic exit of individual Schwann cells (SCs) is essential for radial sorting and peripheral myelination. However, it remains unknown when, during their multiple rounds of division, SCs initiate myelin signaling in vivo. By manipulating SC division during development, we report that when SCs skip their division during migration, but not during radial sorting, they fail to myelinate peripheral axons. This coincides with a sharp decrease in Laminin expression within the posterior lateral line nerve. Interestingly, elevating cAMP levels or forcing Laminin 2 expression within individual SCs restore their ability to myelinate, despite missing mitosis during migration. Our results demonstrate a limited time window during which migrating SCs initiate Laminin expression to gradually activate the Laminin/Gpr126/cAMP signaling required for radial sorting and myelination at later stages in vivo.
Epub:
Not Epub
Link to Publication:
https://www.sciencedirect.com/science/article/pii/S2667290124001037
Organism or Cell Type:
zebrafish
Delivery Method:
microinjection