Citation:
Mol Cells. 2025 Mar 3:100207. doi: 10.1016/j.mocell.2025.100207. Epub ahead of print. PMID: 40043779
Abstract:
Nodal-related ligands of TGF-β family play pivotal roles for mesoderm induction and body axis formation during vertebrate early embryogenesis. Nodal ligands are distinct from most other TGF-β ligands family as they require EGF-CFC factors as co-receptors for signaling, in addition to their cognate type I and type II TGF-β receptors. In amphibian Xenopus laevis embryos, five nodal-related genes (Xnr1/2/4/5/6) and two EGF-CFC genes (XCR1, XCR3) play roles in mesoderm induction and the accumulation of phosphorylated Smad2, while in mammalian embryos one Nodal gene and one EGF-CFC gene (Cripto) play roles during mesoderm induction. Mammalian EGF-CFC factors are reported to be O-fucosylated at a conserved threonine residue of the EGF-like motif by protein O-fucosyltransferase 1 (Pofut1), but this O-fucose modification is shown to be dispensable for Nodal signaling in mammalian embryos. In this study, we investigated the developmental roles of Xenopus laevis Pofut1 (XPofut1) and its potential function in Nodal signaling. We found that morpholino antisense-mediated knockdown of XPofut1 causes reduction of Smad2 phosphorylation in late blastula and axial truncation in neurula. We also found that the O-fucosyltransferase activity of XPofut1 is important in the marginal zone, but not in the vegetal pole region, of blastula. Interestingly, XPofut1 is necessary for Smad2 phosphorylation induced by Xnr1 or Xnr2, but not by Xnr5 or Xnr6. Among the Nodal signaling components, only EGF-CFC factors are known to be modified by Pofut1. Therefore, based on our current observation, we propose that XPofut1 regulates signaling of a subset of nodal ligands in pre-gastrulation embryos possibly through modulating the function of EGF-CFC factors.
Epub:
Not Epub
Link to Publication:
https://www.sciencedirect.com/science/article/pii/S1016847825000317
Organism or Cell Type:
Xenopus laevis
Delivery Method:
microinjection