Citation:
Methods Mol Biol. 2016;1457:209-15. doi: 10.1007/978-1-4939-3795-0_15
Abstract:
Western Blotting has been used extensively for the identification of the protein factors that regulate mammalian oocyte meiosis. However, the limitations in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.
Epub:
Not Epub
Link to Publication:
http://link.springer.com/protocol/10.1007%2F978-1-4939-3795-0_15
Organism or Cell Type:
mouse oocyte