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Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology

Authors: 
Schulte-Merker S, Stainier DY
Citation: 
Development. 2014 Aug;141(16):3103-4. doi: 10.1242/dev.112003
Abstract: 
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
Epub: 
Not Epub