Citation:
Cold Spring Harb Protoc. 2018 Aug 21. doi: 10.1101/pdb.prot097279. [Epub ahead of print]
Abstract:
Introducing exogenous DNA into an embryo can promote misexpression of a gene of interest via transcription regulated by an attached enhancer-promoter. This protocol describes plasmid DNA microinjection into Xenopus embryos for misexpression of genes after zygotic gene expression begins. It also describes a method for coinjecting a reporter plasmid with mRNA or antisense morpholinos to perform luciferase reporter assays, which are useful for quantitative analysis of cis-regulatory sequences responding to endogenous or exogenous stimuli in embryos.
Epub:
Not Epub
Link to Publication:
http://cshprotocols.cshlp.org/content/early/2018/08/21/pdb.prot097279.abstract
Organism or Cell Type:
Xenopus
Delivery Method:
microinjection