Citation:
Methods Mol Biol. 2017;1565:51-57. doi: 10.1007/978-1-4939-6817-6_5
Abstract:
Photoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
Epub:
Not Epub
Link to Publication:
http://www.springer.com/us/book/9781493968152
Organism or Cell Type:
zebrafish
Delivery Method:
microinjection