Citation:
Methods Mol Biol. 2015 Mar 31. [Epub ahead of print]
Abstract:
Morpholino oligonucleotides (MO) are an innovative tool that provides a means for examining and modifying gene expression outcomes by antisense interaction with targeted RNA transcripts. The site-specific nature of their binding facilitates focused modulation to alter splice variant expression patterns. Here we describe the steric-blocking of human telomerase reverse transcriptase (hTERT) Δα and Δβ splice variants using MO to examine cellular outcomes related to pluripotency and differentiation in human embryonic stem cells.
Epub:
Yes
Link to Publication:
http://link.springer.com/protocol/10.1007%2F7651_2015_226
Organism or Cell Type:
cell culture: human embryonic stem cells