Current Methods in Zebrafish Research

Zebrafish (Danio rerio) is a fresh water fish that inhabits rivers in India, Pakistan, and other places in Asia. In the past two decades, Zebrafish has become one of the preferred in vivo model organisms for studying diverse processes like embryogenesis, cell migration, organ formation, behaviour, and sleep. Recently, Zebrafish has sparked interest in other branches of the biomedical research because of its emerging potential for drug discovery in various disease models. For instance, zebrafish with the mutated MYO18B gene can serve as a model for human myopathy [8]. This review addresses a selection of the most commonly used methods in zebrafish research. While the general breeding and handling of zebrafish are detailed in the "Zebrafish Book" [9], this article highlights the important tools and methodology available in zebrafish research such as microinjection, morpholino knockdown, and transplantation, and discusses their applications.